小白菜自交不亲和性相关基因的分子标记开发

以自交亲和系WS-199和自交不亲和系WS-85为亲本杂交获得的F_2分离群体为供试材料,采用BSA法结合SNP芯片技术,筛选出与自交不亲和性相关的SNP标记,并将SNP差异位点序列信息与白菜基因组序列进行比对分析,并进一步开发SSR标记,共获得了与自交不亲和性相关的SSR分子标记BrA1-2、BrA1-3和BrA1-14,为分子标记辅助自交不亲和性杂交种生产提供技术支持。     

物理农业工程技术在南药发展中的应用现状

近年来，物理农业在我国北方地区受到重视，并在一些农业产业投入和运用，其技术价值和优点在生产过程已显示出来。随着物理农业工程技术在国际与国内市场的不断完善和发展，有可能成为我国第四代农业发展方向。以云南为例，分析当前南药发展现状，充分论述利用当地气候资源、地理优势及物理农业工程技术优势发展云南南药的必要性。并预测在发展过程中将要面临的各种困难，最后提出运用物理农业工程技术发展南药产业的建议。     

Study on Liver-protective Effect of Chinese Herbal Compound Probiotics on Acute Liver Injury Layers

This experiment was conducted to investigate the liver-protective effect of Chinese herbal compound probiotics (CHCP) on acute liver injury layers.One hundred and eight 1 day old hens were divided into 4 groups with 3 replicates in each group and 9 layers per replicate in trial 1.The layers in model groups Ⅰ to Ⅲ were gavaged with 10% (V/V) soybean oil solution of carbon tetrachloride (SCCl₄) according to 1,2 and 4 mL/(kg·BW) at 14,28 and 35 d,respectively.The layers in control group were gavaged with 2 mL/(kg·BW) soybean oil.In trial 2,sixty 1 d layers were divided into 5 groups:Control group (soybean oil),model control group(SCCl₄)and low-dose,middle-dose and high-dose CHCP group (SCCl₄+1‰ CHCP,SCCl₄+2‰ CHCP and SCCl₄+4‰ CHCP respectively).CHCP were used by drinking water since 7 days.SCCl₄ were gavaged according to 2 mL/(kg·BW) at 14 and 28 d.The results showed as follows:The model of layers liver damage could be built by intragastric administration of 2 mL/(kg·BW) 10%(V/V) SCCl₄ at 14,28 d respectively,with the signs of hepatic steatosis,severe vacuolar degeneration,nuclear condensation and necrosis.Compared to the model control group,the serum AST levels in low,medium and high dose CHCP groups were decreased by 4.35% (P > 0.05),7.57% (P > 0.05) and 9.79% (P < 0.05),the serum ALT levels in medium and high dose CHCP groups were decreased by 34.92% (P < 0.01),36.51% (P < 0.01),the serum total bilirubin content in medium and high dose CHCP groups were decreased by 25.49% (P < 0.01),27.45% (P < 0.01).The liver cell congestion was reduced to varying degrees in different dose CHCP groups,and the liver cell had no vacuoles,arranged in neat rows,abundant cytoplasm and uniform in different dose CHCP groups.In conclusion,2‰,4‰ CHCP could reduce hepatocyte necrosis,decrease the serum activities of ALT,AST and total bilirubin levels,and had protective effect on hepatic injury induced by SCCl₄.     

中晚熟大白菜新品种天白80的选育

天白80是以雄性不育系C441和自交不亲和系T01配制而成的中晚熟直筒型大白菜一代杂种,生长期75d(天)左右,株型直立,株高51cm,开展度58.5cm,外叶浅绿色,叶面少茸毛,中肋白色;叶球长筒形,顶部舒心,球色浅绿,结球紧实,内叶浅黄色,球高46.4cm,球径11.2cm,球形指数4.1;叶球质量2.8kg左右,净菜率75.4%。抗病性强,高抗病毒病和霜霉病,抗黑腐病。平均每667m2产量7200kg左右,适宜我国华北及西北地区作为中晚熟秋大白菜种植。     

Molecular cloning and expression of a novel eukaryotes elongation factor1A gene (ZjeEF-1α) from Chinese jujube in response to phytoplasma infection

Eukaryotic translation elongation factor 1A (eEF1A) in plant is a multifunctional protein. In our previous study, we identified a 651-bp EST sequence of eEF-1α from a suppression subtractive hybridization (SSH) library of Chinese jujube (Ziziphus jujuba Mill.), which was established under jujube witches' broom (JWB) phytoplasma stress. Here we isolated the eEF1A gene (ZjeEF-1α, JF488063) from Chinese jujube by single oligonucleotide nested (SON)-PCR and rapid amplification of cDNA ends (RACE). ZjeEF-1α contains an open reading frame (ORF) of 1344 bp, encoding a polypeptide of 447 amino acids with an estimated molecular mass of 49.29 kDa and a theoretical pI of 9.10. ZjeEF-1α shared over 92% similarity with eEF-1α proteins from other 24 eukaryotes including plants and animals. Search Pfam predicted that ZjeEF-1A contains three typical eEF1A functional domains. Moreover, the corresponding genomic sequence of ZjeEF-1α was also sequenced, in which a 115-bp intervening sequence with splicing site GT-AG was found. Real-time quantitative PCR (RT-qPCR) and western blotting assays revealed that the expression of ZjeEF-1α was enhanced in resistant cultivar ‘Xingguang’ and decreased in sensitive cultivar ‘Zanhuangdazao’ at early stages of phytoplasma infection. It is proved that ZjeEF-1A expression is early response to phytoplasma infection and its possible functions in resistant process to JWB phytoplasma are discussed.     

中医理论在园林景观中的应用类型探析

以中医学、生态学、景观美学和环境心理学为理论基础，研究了中医理论在园林景观中的应用类型及各应用类型之间的异同，总结了中医理论在园林景观中的应用方式，并分析了目前中医理论在园林景观中应用时存在的不足，以期为中医理论在园林景观中的应用提供理论依据。     

抑制鹿源多药耐药结核菌中药的筛选

为了筛选对鹿源多药耐药结核菌有效的中药耐药抑制剂,通过MABA法检测24种中药的水和乙醇提取物对多药耐药结核菌及标准菌H37Rv的体外抗菌效果,将筛选出的抗菌效果好的黄连醇提物和黄连水提物采用紫外分光光度法进行活性成分含量测定,同时以MABA法与标准品比较体外抗结核菌活性。结果显示,黄连、独活和侧柏叶有明显的抗结核活性,其中黄连水粗提物和乙醇粗提物效果最佳,测得它们活性成分即总生物碱含量分别为22.74%和35.23%,并显示二者与小檗碱的体外抗结核作用相同。     

基于信号博弈的阳澄湖大闸蟹绿色标签市场应用分析

水产品绿色标签对消费者来说是高质量的信号,对卖家来说则是卖出高价的资本.但是欺诈行为的存在损害了消费者和其他卖家的利益,扰乱了市场秩序.以阳澄湖大闸蟹为例,通过信号博彝理论,对螃蟹市场中关卖双方的消费行为进行博彝分析,得到了精炼贝叶斯Nash均衡.     

用户行为驱动的网页布局自动调整的研究

逻辑观是困扰当前中国逻辑史研究的根本性问题。两千多年来，逻辑的名称虽几经变化，但对命题形式、推理形式及其有效性的分析，始终是西方逻辑不变的主题。由于中国逻辑史研究是基于西方逻辑的传入而产生的，因此，西方逻辑的逻辑观应当成为中国逻辑史研究的基本遵循。     

绵羊FGF10基因克隆、序列分析及其在毛囊发育中的表达分析

本试验旨在获得中国美利奴羊成纤维细胞生长因子10（fibroblast growth factor 10，FGF10）基因的编码区（CDS）全长序列并进行生物信息学分析，随后对FGF10基因在中国美利奴羊毛囊发育过程中的表达特征进行分析，明确其在中国美利奴羊毛囊发育过程中的表达模式，为进一步研究FGF10 mRNA表达水平与中国美利奴羊毛囊生长发育的表达调控机制奠定理论基础。采用PCR扩增获得中国美利奴羊FGF10基因CDS，并克隆到zero PCR@^TM-Blunt进行测序验证；利用实时荧光定量PCR技术检测FGF10在中国美利奴羊毛囊发育过程中的表达差异。结果表明，绵羊FGF10基因CDS长度为696 bp（序列上传GenBank，获得登录号：MT872422），编码231个氨基酸，与牛和山羊的氨基酸序列同源性达100%，存在1个信号肽和1个跨膜结构域，其为分泌通路信号蛋白；实时荧光定量PCR分析表明，FGF10基因在中国美利奴羊毛囊发育过程中均表达，在毛囊发育第85天表达最高，显著高于其他毛囊发育时期（P<0.05）。本研究获得中国美利奴羊FGF10基因完整的编码区序列和毛囊发育过程中的表达特征，生物信息学分析发现，FGF10基因编码区序列具有物种间的保守性，同时FGF10在绵羊毛囊不同发育阶段的皮肤组织中表达，由此表明，FGF10基因可能在绵羊毛囊的生长发育过程中发挥重要的生物学作用。